Enzymatic Neutralization of the Chemical Warfare Agent VX: Evolution of Phosphotriesterase for Phosphorothiolate Hydrolysis

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Enzymatic neutralization of the chemical warfare agent VX: evolution of phosphotriesterase for phosphorothiolate hydrolysis.

The V-type nerve agents (VX and VR) are among the most toxic substances known. The high toxicity and environmental persistence of VX make the development of novel decontamination methods particularly important. The enzyme phosphotriesterase (PTE) is capable of hydrolyzing VX but with an enzymatic efficiency more than 5 orders of magnitude lower than with its best substrate, paraoxon. PTE has pr...

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While neurobehavioral effects of acute exposure to toxic levels of chemical warfare nerve agents (CWNA) have been characterized (e.g. [8]), much less is known about the effects of repeated exposure to non-convulsive levels of CWNA. In Exp. 1, male mice that received repeated exposure (1/day x 5 days/wk x 2 wk) to 0.4 LD50 of the nerve agent VX had much lower activity in the home cage, relative ...

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ژورنال

عنوان ژورنال: Journal of the American Chemical Society

سال: 2013

ISSN: 0002-7863,1520-5126

DOI: 10.1021/ja402832z